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KMID : 0385019980140020219
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Korean Journal of Laboratory Animal Science
1998 Volume.14 No. 2 p.219 ~ p.227
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Studies on the Basic Data of Ktc:Nude Mice with Age
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조규혁/Cho, Kyu Hyuk
양승돈/백승규/임광현/송창우/한상섭/Yang, Sung Don/Paik, Seung Kyu/Lim, Kwang Hyun/Song, Chang Woo/Han, Sang Seop
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Abstract
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The athymic $quot;nude$quot; mouse is an useful animal model for the study of cell-mediated immunologic deficiencies and for assaying the neoplastic growth potential in vivo of animal cells transformed in vitro. We monitored the changes, with age, of weights (body and various organs), physiological parameters (hematology, blood and urine analysis), and lymphocyte populations (only one time point, 10 weeks of age) in spleen of BALB/cAnNCrj-nu mice, and the parameters were compared to those of their counterparts (BALE/cAnNCrj) which were used as breeding stocks of BALB/cAnNCrj-nu mice production. Some of the parameters were compared between males and females. Body weights of BALB/cAnNCrj-nu mice were always lighter than those of counterparts in both males and females. The differences in body weights between seates were more distinct in BALB/cAnNCrj than in BALB/cAnNCrj-nu mice. The weights of most organs were not significantly different between BALB/cAnNCrj-nu and wild type. Spleen weights, however, showed different patterns between the groups. At 5 weeks of age, BALB/cAnNCrj-nu mice showed lighter spleens than those of wild types. The weights increased gradually with age and eventually exceeded the weights of the wild type spleens either at 10 or 18 weeks of age. The spleen lymphocytes were more dramatically different between the groups. Up to 83% of spleen cells in BALB/cAnNCrj-nu mice were surface Ig^+ cells, implying that most of them are B lymphocytes. CD4^+ cells were negligible (less than 510) in BALB/cAnNCrj-nu mice as anticipated. This phenomenon implies that T lymphocytes were not differentiated in BALB/cAnNCrj-nu mice due to absence of distinct thymus. In wild type, Ig^+ cells were approximately 55% and the rest were CD4^+ cells. Other various parameters of hematology, blood and urine biochemistry showed in normal range suggesting that the animals subjected in the experiments, and thus the animals in our breeding colony, are in good health. Thus, we provide the basic parameters of BALB/cAnNCrj-nu mice, and these data will serve as the basic information of BALB/cAnNCrj-nu mice bred in our colony.
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